Final Diagnosis & Discussions: EUS FNA of a Gastric Mass in a 61-Year-Old Male

FINAL DIAGNOSIS: Gastrointestinal Stromal Tumor (GIST)

 

DISCUSSION
Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors of the gastrointestinal tract. The majority of these tumors are found in the stomach. The cell of origin is considered to be the intestinal pacemaker cell, the cell of Cajal. GISTs are usually accompanied by a somatic mutation of CD-117(c-kit), which is a tyrosine kinase receptor normally expressed by the intestinal cells of Cajal. The specific diagnosis of GIST is important because of the possible response to tyrosine kinase inhibitor, imatinib mesylate (Gleevec).

GISTs show two basic cells types: spindle and epithelioid. Epithelioid variants occur in approximately 25-30% of gastric GISTs and correspond to tumors previously termed “leiomyoblastomas” or “epithelioid leiomyosarcoma”.

The cytology depends upon the cell type, but there are no pathognomonic cytologic features of GIST. In epitheliod GISTs, cytologic smears are usually cellular, and the cells appear predominantly singly or in loose clusters. Upon processing with Papanicolaou stain, the epitheliod cells contain moderate amount of amphophilic to eosinophilic cytoplasm. Nuclei are round or oval and vesicular. Intranuclear inclusions and binucleation are frequent findings. Small nucleoli may also be present. Stroma is usually myxoid and transgressing capillaries may be seen.

The most important immunocytochemical marker of GIST is expression of c-kit (CD117), which is found in vast majority (>90 percent) of GISTs. Staining is usually strong and widespread. It may show a variety of patterns including diffuse cytoplasmic staining, localized perinuclear dote and membranous.

Most (>80 percent) gastric GISTs also express CD34, but smooth muscle actin is demonstrable in about 25 percent.

Some GISTs can be “KIT-negative”, despite having typical morphological (and other immunohistochemical) features.

This is an unusual case of epithelioid variant of GIST which was negative for c-kit (CD117) on both FNA and surgical resection. Significant pitfalls for the diagnosis of epitheliod GIST include neuroendocrine tumors, carcinomas and melanomas, and other neoplasms with an epitheliod appearance. Differential diagnosis with neuroendocrine tumors may be challenging because of the plasmacytoid appearance of cells along with round nuclei containing finely granular chromatin. Immunocytochemical analysis (with chromogranin or synaptophisin) can also be helpful in determining the diagnosis.

When the GIST is composed of discohesive tumor cells with eccentric large nuclei, prominent nucleoli, and intranuclear inclusions, melanoma becomes an important differential diagnosis. However GISTs are usually negative for the common melanoma markers such as S-100, Mart-1, and HMB-45.

While it is prudent for a cytopathologist to consider GIST in the differential diagnosis of any unusual gastric neoplasm with epithelioid features, it is important to realize that not all GISTs are c-kit (CD-117) positive.

References

  1. WHO Classification of Tumours of Digestive System: IARC Press, Lyon, 2000.

  2. DeMay M. Practical Principles of Cytopathology. 2007.

  3. Dong Q, McKee G, Pitman M, Geisinger K, Tambauret R. Epithelioid variant of gastrointestinal stromal tumor: Diagnosis by fine needle aspiration. Diagnostic cytopathology. 29(2):55-60.

  4. Lograno R, et al. Imaging, morphologic, and immunohistochemical correlation in gastrointestinal stromal tumors. Cancer Cytopathology. 2006;June:257-267.