WEBVTT

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In traditional microbiology,

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streaking is a technique
used to isolate microorganisms.

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The process is done
manually using a sterile tool,

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such as a metal or plastic inoculation
loop.

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Aseptic techniques are used to maintain
microbiological cultures

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and to prevent contamination
of the growth medium.

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Let's plate a urine culture manually

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using the sheep blood agar plate.

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First, hold a plastic one

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microliter calibrated loop vertically

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and immerse
it just below the surface of the urine.

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Ensure
that the urine completely fills the loop.

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Second, streak it
vertically down the center of the plate.

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Third, take the loop again on its edge
and make a zigzag motion

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roughly ten times across the streak
of urine along the plate.

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Manual plating can be very labor intensive
with instrumentation.

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Plating can be done
using the automated processor.

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The instrument can process positive blood

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cultures, tissues, wound aspirates,

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body fluids,
and traditional swab and swab samples.

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Each swab

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allows the entire sample
to be transferred into a liquid medium,

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providing eloquence

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of a liquid sample suspension,
which can be used for multiple tests.

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The automated specimen processor
has the ability to fully automate

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all aspects of microbiology processing,
including plating, streaking,

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gram stain, slide preparation
and enrichment, broth inoculation.

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The user can customize the streaking
patterns to ensure optimal isolation.

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Reusable loops
help keep the operational cost low

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and include one Microliter,
ten Microliter,

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and 30 Microliter volumes, which provide

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precise volumes for quantitative analysis.

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Gram stain slides are also prepared
and labeled automatically

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with increased workloads
and labor shortages.

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An automated microbiology
processor is a clever innovation

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that can be a cost effective process
to address an influx of samples.

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The classic way of reading cultures
is based on certain time frames.

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For example, standard reading times
for optimal growth of urine specimens

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are at 18 to 24 and 48 hours
from the setup time.

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Laboratory professionals
performed all the reading,

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workup and entering of the results.

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This traditional method of testing allows
labs to make accurate workup decisions.

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However, it takes time to sort
the negative from the positive plates.

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The manual process may delay
the patient results as plates

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are only read
periodically throughout the day.

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Automated

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microbiology technology allows laboratory
professionals to read the cultures

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by using a camera system
to capture images at defined intervals.

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Upon entering the incubator,

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the camera takes
an initial photo of the plate.

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Then, based on user defined software,

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it will continue to take pictures
of the plates at the programed times.

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Images are stored and used

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for quality assurance purposes.

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Let's look at the urine samples.

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Urine cultures are categorized based on
colony counts with growth no growth.

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Discrimination.

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The software will automatically sort out
no growth cultures

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and can result up to 30 negative samples
at once.

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Technologists never touch a negative plate

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with a click of a send button.

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The instrument will show final no growth

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and discards the negatives quickly.

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The software integrates
with the labs to provide quick,

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accurate results reported to clinicians
for timely patient care.

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Plates that are ready to

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read are displayed on the dashboard.

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Plates that require further investigation

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are displayed in the screening area
on the computer monitor.

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Users can zoom in on the plate images
for a better view of colonies.

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Users hit the investigation button

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to request presumptive positive plates
from the canisters,

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and bring them to the bench
for further testing.

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Technologists
manually read plates when they are ready,

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which improves the turnaround time.

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There may be some challenges with
digital microbiology when problems arise.

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Errors in the system can negatively impact
the positive strides

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the technology makes when it works
the way it should.

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The instrument may shut down
due to errors, or on occasion,

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it may crush the plates,
or the barcode station may have a problem

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scanning the plates. Labels.

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However, when it is functioning properly,

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the software automatically recognizes
the presence of organisms,

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allowing microbiology labs
to quickly read, interpret,

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and segregate bacterial cultures
with the click of a button.