WEBVTT

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Performance of the acid test is done
to confirm the initial positive

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ketone results from the reagent dipstick,
and to semi quantitatively

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measure the concentration of ketone bodies
in the urine.

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The performance of the acid test
will be demonstrated using positive

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and negative control specimens.

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Positive identification of a specimen
must be maintained

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throughout the complete testing process.

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Supplies needed to perform
this test include paper towels,

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a permanent marker, tweezers, disposable
pipettes,

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a timer, a bottle of acid test tablets,
and the manufacturer's color chart.

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The test is performed on a clean, dry
paper towel using a permanent marker

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label.

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The area of the paper towel
you will use for each specimen

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using clean, dry tweezers, remove one

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acid test
tablet per specimen from the bottle

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and place it onto the paper towel
in the labeled areas.

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Acid test tablets are sensitive
to light, heat, and moisture,

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so the bottle needs to be recapped
immediately

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after removing a tablet
and when not in use.

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Also, do not touch the tablets
with anything but the tweezers.

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The specimen must be thoroughly mixed
by gently

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inverting the container
a minimum of eight times.

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Carefully remove the cap from the specimen
container and place it

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specimen side up onto the benchtop
using a disposable pipette.

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Gently dispense one drop of the urine
specimen onto the tablet,

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and then dispose of the pipette
into a bio hazardous waste receptacle.

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Immediately start a timer for 30s.

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Recap the specimen at 30s.

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Compare the color of the tablet

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against the manufacturer's color chart
and record the results as negative.

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Small, moderate, or large.

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Discard the paper towel and tablet
into a bio hazardous waste receptacle.