WEBVTT

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The cleaning

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test is performed
on all pediatric specimens to aid

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in the early diagnosis of inborn errors
of metabolism, such as galactose anemia.

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Each facility will have its own protocol
regarding the performance of this test.

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The performance of the clinical

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test will be demonstrated using positive
and negative control specimens.

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Positive identification of a specimen
must be maintained through the complete

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testing process.

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Supplies needed to perform
this test include

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paper towels, a test tube rack,

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clean dry test tubes, tweezers,

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a permanent marker, disposable pipettes,

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laboratory
grade water clinic, test tablets,

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and the manufacturer's color chart
using a permanent marker

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label one test tube for each specimen
to be tested thoroughly mixed the specimen

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by gently inverting the container
a minimum of eight times.

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Remove the cap from the specimen

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container and place its specimen
side up onto the benchtop.

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Using a disposable pipette, dispense

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five drops of the urine specimen
into the appropriate test tube.

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Dispose of the pipette
into a bio hazardous waste

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receptacle using a disposable pipette.

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Dispense ten drops of laboratory
grade water into the test tube

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containing the urine specimen.

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Dispose of the pipette
into a bio hazardous waste receptacle.

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Using clean, dry tweezers,
retrieve one and test tablet per specimen

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from the bottle and gently
drop it into the liquid in the test tube.

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Be careful as the addition of the tablet
to the liquid causes

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an exothermic reaction in the test tube,
and generates very high temperatures.

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Note that the specimen boils
within the tube.

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If this reaction is not observed,

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the clinical test tablets
may not be working properly.

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Clinical test tablets are sensitive
to light, heat, and moisture,

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so the bottle needs to be capped
and sealed immediately

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after removing a tablet
and when not in use.

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Also, do not touch the tablets
with anything but the tweezers.

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Recap the specimen watch
while the boiling reaction takes place.

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Be careful not to disturb the test tube
during the reaction.

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Once the boiling has ceased, start
a timer for 15 seconds.

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Do not disturb the tube during this 15
second incubation

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time after the 15 second incubation.

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Gently shake the test tube to mix
the contents of the tube.

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Compare the color of the solution
against the manufacturer's color chart,

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while ignoring any sediment that may have
formed at the bottom of the tube.

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Record the results
as indicated on the color chart.

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Discard the reaction test tube
into a bio hazardous waste receptacle.