﻿WEBVTT

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The sulfur salicylic acid test, or SSR

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test is done to confirm the presence
or absence of protein in the urine.

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Depending on the facility's protocol,
this test may be performed to confirm

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positive or negative protein results
from the initial screening test.

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This test semi quantitatively measures
the concentration of protein in the urine.

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Positive identification of a specimen
must be maintained through the complete

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testing process.

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Supplies needed for this test
include a test tube rack,

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clean dry test tubes, a bottle of 20% SSR,

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and an adjustable pipette with tips
to deliver one milliliter

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using a permanent marker label one test

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tube for each specimen tested.

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The specimen must be thoroughly mixed
by gently

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inverting the container
a minimum of eight times.

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Remove the cap from the specimen
container and place

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at specimen side up onto the benchtop.

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Add one milliliter of urine specimen
to a clean, dry test tube.

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Dispose
of the pipette tip into an appropriate

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bio hazardous waste receptacle.

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Recap the specimen using a new pipette tip
at approximately one

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milliliter of 20% SSR to the tube,
for a total volume of two milliliters.

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These measurements
do not have to be exact,

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but there should be equal volumes
of urine.

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An SSR dispose of the pipette tip
into a appropriate bio

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hazardous waste receptacle.

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Gently shake the tube or tubes
to completely mix the specimen

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and SSR solution.

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Using a dark background may be helpful
when grading the reaction.

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Grade the appearance of the solution
and report the results.

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Discard the test tube into a bio
hazardous waste receptacle.
