﻿WEBVTT

00:00:00.083 --> 00:00:01.793
Hello, my name is Madison Sant

00:00:01.793 --> 00:00:04.796
and I'm from Mary Pugh Laboratories
parasitology department.

00:00:05.046 --> 00:00:08.383
This presentation will cover blood
parasites and queen Trypanosoma.

00:00:08.591 --> 00:00:11.011
Leash mania and microflora.

00:00:11.011 --> 00:00:14.639
Please fill out the Parasitology Practice
worksheet while listening to the lecture.

00:00:15.098 --> 00:00:16.391
Feel free to pause the lecture.

00:00:16.391 --> 00:00:17.851
Take your notes.

00:00:17.851 --> 00:00:19.936
Write down any questions you may have

00:00:19.936 --> 00:00:22.814
so that we can discuss them
when we meet in person.

00:00:22.814 --> 00:00:24.524
If we will not be meeting in person.

00:00:24.524 --> 00:00:27.610
I was just talking to professor
about any questions you may have.

00:00:30.071 --> 00:00:32.323
The organisms we will
discuss are Trypanosoma

00:00:32.323 --> 00:00:35.326
leash, mania and microflora.

00:00:36.870 --> 00:00:38.538
The stain commonly used to detect

00:00:38.538 --> 00:00:41.624
other blood parasites is a glimpse unseen,
thick and thin.

00:00:41.624 --> 00:00:44.586
Smears are made from whole blood and EDTA.

00:00:44.669 --> 00:00:47.672
The thick smears are used to detect
the presence of the parasite,

00:00:47.881 --> 00:00:51.259
but the thin smears
I use for species level identification.

00:00:53.720 --> 00:00:54.846
We will discuss two

00:00:54.846 --> 00:00:58.808
different types of Japan isms
Japan's democracy and Japan's somewhat.

00:00:58.808 --> 00:01:01.811
Bruce, I.

00:01:02.312 --> 00:01:05.315
Trypanosoma cruzi causes Chagas disease,

00:01:05.356 --> 00:01:08.359
which is endemic to the American tropics,

00:01:09.027 --> 00:01:10.361
and infected trattamento.

00:01:10.361 --> 00:01:15.116
The insect vector or kissing bug
takes a blood meal and releases two Tomasi

00:01:15.116 --> 00:01:18.912
goats in his feces and the other side
of the bite wound to Damascus.

00:01:18.912 --> 00:01:20.622
Enter the host, the bite wound,

00:01:20.622 --> 00:01:24.793
or through a causal membrane
such as the conjunctiva inside the host.

00:01:24.793 --> 00:01:28.004
The true Tomasi goats invade cells
near the site of an occupation,

00:01:28.379 --> 00:01:31.382
where they become intracellular MRC goats.

00:01:31.382 --> 00:01:36.805
The MRC goats multiply by binary fission
and become true to Marcy goats, which are

00:01:36.805 --> 00:01:40.600
then released into the circulation
as blood stream two to Marcy goats.

00:01:41.351 --> 00:01:44.354
Two domestic goats
infect cells of various tissues

00:01:44.604 --> 00:01:48.316
and transform into intracellular
MASC goats and new infection sites.

00:01:48.775 --> 00:01:52.153
The kissing
bug becomes infected by feeding on a human

00:01:52.153 --> 00:01:55.573
or animal blood
that contains circulating parasites.

00:01:56.241 --> 00:01:59.619
The ingested trick to Marcy goats
transform into epi Marcy goats.

00:01:59.828 --> 00:02:03.373
In the vectors
mid gut, the parasites become infected.

00:02:03.373 --> 00:02:06.376
Made a cyclic trip
to Marcy goats in the hind gut.

00:02:06.751 --> 00:02:10.213
Other less common routes of transmission
include blood transfusions,

00:02:10.505 --> 00:02:15.426
organ transplantation,
transpersonal transmission, and foodborne

00:02:15.426 --> 00:02:19.472
transmission by food or drink contaminated
with the kissing bug and its feces.

00:02:20.306 --> 00:02:23.476
Chagas disease has an acute phase
and a chronic phase.

00:02:23.893 --> 00:02:28.648
The acute phase is usually asymptomatic,
but can present with nonspecific symptoms

00:02:29.315 --> 00:02:31.776
nodular lesions or for ankles,

00:02:31.776 --> 00:02:35.363
usually called a gomez, may develop
around the vectors.

00:02:35.363 --> 00:02:37.031
Feeding site to Gomez.

00:02:37.031 --> 00:02:40.326
Occurring on the eyelids
are commonly referred to as romana sign.

00:02:40.994 --> 00:02:44.622
The chronic phase may include cardiac
and gastrointestinal involvement.

00:02:45.248 --> 00:02:48.751
A mouse to go an invasion of smooth muscle
can lead to mega

00:02:48.793 --> 00:02:52.839
esophagus, mega colon,
and dilated cardiomyopathy.

00:02:53.590 --> 00:02:56.676
Complications
of chronic Chagas disease may be fatal.

00:02:59.429 --> 00:03:00.013
Unless

00:03:00.013 --> 00:03:03.016
you go to Japan of Samoa, cruzi
can be found a tissue

00:03:03.016 --> 00:03:06.477
and have one nucleus and can a plus two.

00:03:06.477 --> 00:03:06.936
Demasi.

00:03:06.936 --> 00:03:09.939
Goats are 12 to 30 microns
and have a large candida,

00:03:09.939 --> 00:03:13.776
plus a center located nucleus
and undulating membrane.

00:03:14.068 --> 00:03:15.737
And if a germ running along

00:03:15.737 --> 00:03:18.740
the anjali membrane,
leaving the body at the anterior end.

00:03:19.616 --> 00:03:23.328
Tritium associates can be seen
in peripheral blood and a cerebral spinal

00:03:23.328 --> 00:03:26.331
fluid and central nervous system
infections.

00:03:27.415 --> 00:03:30.919
African Japan isms include two subspecies
that are morphologically

00:03:30.919 --> 00:03:34.464
indistinguishable to Pam soma Bruce,
examining C

00:03:34.714 --> 00:03:39.093
causing chronic African trypanosomiasis
or West African sleeping sickness,

00:03:39.636 --> 00:03:42.889
and Trypanosoma Bushrod NC causing acute

00:03:42.889 --> 00:03:46.726
African trypanosomiasis
or East African sleeping sickness.

00:03:47.560 --> 00:03:52.148
Trypanosoma cruzi gamma
C is endemic in West and Central Africa.

00:03:52.815 --> 00:03:56.319
Sarah Zelma
Bruce Girard, NC is restricted to east

00:03:56.319 --> 00:03:59.405
and Southeast Africa during a blood mill

00:04:00.531 --> 00:04:02.533
and infected CC fly insects.

00:04:02.533 --> 00:04:05.536
Melissa
Cook To see mousey goats into skin tissue.

00:04:05.954 --> 00:04:08.456
The parasites enter the lymphatic system

00:04:08.456 --> 00:04:11.501
and pass into the bloodstream
inside the host.

00:04:11.501 --> 00:04:14.045
They transformative bloodstream
to domestic goats,

00:04:14.045 --> 00:04:16.297
which are carried to other sites
throughout the body

00:04:16.297 --> 00:04:19.717
and reach other body fluids,
including lymph and spinal fluid.

00:04:20.176 --> 00:04:23.179
The trip to Marcy goats
replicate by Bernard fission.

00:04:23.429 --> 00:04:26.766
The CC fly becomes infected
with bloodstream to domestic goats

00:04:26.766 --> 00:04:29.769
when taking a blood meal on an infected
human host,

00:04:30.228 --> 00:04:32.272
and the flies may gut the parasite.

00:04:32.272 --> 00:04:36.651
Transform into pro cyclic to Tomasi goats
multiply by binary fission,

00:04:37.026 --> 00:04:41.614
leave the maggot, and transform
an epi marcy goats that b marcy goats

00:04:41.614 --> 00:04:45.910
fruits to the fly silver glands
and a t multiplication by binary fission.

00:04:47.245 --> 00:04:50.832
Infection occurs in two stages,
which may sometimes be preceded

00:04:50.832 --> 00:04:52.166
by a development of a shrinker

00:04:52.166 --> 00:04:55.878
at the site of inoculation within days
of being bitten by the infected fly.

00:04:56.713 --> 00:05:00.008
First stage disease involves
nonspecific signs and symptoms

00:05:00.008 --> 00:05:03.011
such as intermittent fever
and lymphadenopathy.

00:05:03.428 --> 00:05:06.639
Winterbottom's sign,
which is the enlargement of cervical lymph

00:05:06.639 --> 00:05:09.100
nodes, is commonly seen in Japan. Samoa,
Bruce.

00:05:09.100 --> 00:05:11.019
Examining and see, and.

00:05:11.019 --> 00:05:15.023
The second stage disease
invasion of central nervous system causes

00:05:15.023 --> 00:05:19.652
a variety of neuropsychiatric
manifestations, including sleep disorders.

00:05:21.487 --> 00:05:22.447
Trypanosoma

00:05:22.447 --> 00:05:27.076
Bruce said to Timothy, goats have a small
native place located the posterior end,

00:05:27.618 --> 00:05:29.579
essentially located nucleus

00:05:29.579 --> 00:05:33.583
and until a membrane and a full German
running along the membrane,

00:05:33.583 --> 00:05:36.336
leaving the body of the anterior
end toward the mouth.

00:05:36.336 --> 00:05:41.049
Seals are the only stage found in patients
and range from 14 to 33 microns.

00:05:42.300 --> 00:05:45.303
Next, we'll discuss leash mania.

00:05:46.679 --> 00:05:47.555
Leash analysis

00:05:47.555 --> 00:05:50.558
is found in parts of about 88 countries.

00:05:50.558 --> 00:05:54.312
Most of the affected countries
are in the tropics and subtropics.

00:05:54.729 --> 00:05:58.232
Leishmaniasis is transmitted
by the bite of infected sand flies.

00:05:58.816 --> 00:06:03.488
The sand flies inject the infective stage
Pro Max egos during blood mills.

00:06:03.863 --> 00:06:06.866
Pro Max seagulls
I reach the puncture wound

00:06:06.908 --> 00:06:09.702
or phagocytosis by macrophages

00:06:09.702 --> 00:06:13.831
Pro Max seals, transforming these cells
into tissue stage or amass goats

00:06:14.207 --> 00:06:17.210
which multiply and proceed
to infect other macrophages.

00:06:17.835 --> 00:06:21.756
Sand flies become infected by ingesting
infected cells during blood mills.

00:06:22.298 --> 00:06:25.051
In sand flies,
a mosquito transformative Pro

00:06:25.051 --> 00:06:28.930
Max develop
in the gut and migrates to the proboscis.

00:06:30.264 --> 00:06:33.226
These
menaces has three main clinical syndromes

00:06:33.226 --> 00:06:37.480
cutaneous, mucosal, and visceral
infections can result in two main

00:06:37.480 --> 00:06:41.567
forms of disease cutaneous analysis
and visceral placement ISIS

00:06:41.567 --> 00:06:44.612
or Kelly also cutaneous tasteless mice.

00:06:44.612 --> 00:06:45.863
This is characterized by one

00:06:45.863 --> 00:06:49.117
or more cutaneous lesions on areas
where sand flies have fed.

00:06:49.784 --> 00:06:53.287
Persons who have continuously surmises
have one or more sores in their skins

00:06:54.038 --> 00:06:55.331
person to have visceral these

00:06:55.331 --> 00:06:58.584
for analysis have fever, weight loss,
and a large spleen and liver.

00:06:59.377 --> 00:07:03.089
Leishmania, a masai goats can be recovered
from aspirin's lesion

00:07:03.089 --> 00:07:07.301
punch biopsies or bone stain
or bone marrow and stain with game sustain

00:07:08.052 --> 00:07:12.765
blood is not the specimen, a choice for
which many detection MRSA gets released.

00:07:12.765 --> 00:07:15.726
Media are round to oval
and measure 1 to 5 microns.

00:07:16.185 --> 00:07:20.314
They have a large nucleus and a prominent
kidney to, plus the organisms

00:07:20.314 --> 00:07:23.776
reside in macrophages of the host
and can be found throughout the body.

00:07:27.613 --> 00:07:29.031
Now we're going going.

00:07:29.031 --> 00:07:31.617
Sorry.

00:07:31.617 --> 00:07:34.662
Now we're going to briefly discuss
the most frequently encountered

00:07:34.662 --> 00:07:37.874
microbial area with Sharia Brazil lower,

00:07:37.874 --> 00:07:40.877
lower and manzanilla.

00:07:42.128 --> 00:07:44.755
Microflora are vector borne diseases.

00:07:44.755 --> 00:07:48.009
The vector injects the larvae
into the skin where it evolves

00:07:48.009 --> 00:07:50.011
into adults and various body sites.

00:07:50.011 --> 00:07:53.347
The location of which depends
on the species adults produce.

00:07:53.848 --> 00:07:56.851
Area that can be found in
peripheral blood.

00:07:57.059 --> 00:07:57.727
Wash area.

00:07:57.727 --> 00:08:01.272
Bancroft dye is found in tropical
and subtropical regions.

00:08:01.689 --> 00:08:05.026
It is transmitted by Anopheles, cox
and 80s mosquitoes.

00:08:05.526 --> 00:08:08.196
Symptoms
include brain coughing or lymphatic

00:08:08.196 --> 00:08:11.199
filariasis,
which may progress elephantiasis.

00:08:11.741 --> 00:08:15.203
Bruja Mali is found in India, Asia,
and Southeast Asia.

00:08:15.995 --> 00:08:19.081
It is transmitted by man Sani, Anopheles
and Kuki.

00:08:19.123 --> 00:08:20.583
Looks mosquitoes.

00:08:20.583 --> 00:08:24.962
Symptoms are similar to a shorter
Bancroft Day, but are often milder.

00:08:25.880 --> 00:08:28.758
Lower Lower East and central West Africa

00:08:28.758 --> 00:08:31.052
is transmitted by mango flies and deer
flies.

00:08:31.052 --> 00:08:35.223
The Christ species and symptoms
include migratory angioedema

00:08:35.431 --> 00:08:39.393
caused by adults moving about
and subcutaneous tissue, but lower

00:08:39.435 --> 00:08:43.814
also causes eye worm, which is where adult
worms are covered from the conjunctiva.

00:08:45.066 --> 00:08:46.692
Manzanillo Sands is found.

00:08:46.692 --> 00:08:51.280
Africa, the West Indies and central South
America is transmitted by biting.

00:08:51.280 --> 00:08:52.907
Midges are the color coded.

00:08:52.907 --> 00:08:57.912
These species symptoms include migratory
angioedema, pericarditis,

00:08:58.329 --> 00:09:01.332
and adult worms reside in body cavities.

00:09:02.166 --> 00:09:03.751
National anxiety

00:09:03.751 --> 00:09:07.255
is found in Central
and South America, is transmitted by body.

00:09:07.255 --> 00:09:11.509
Midges are the killer coyote species
and black flies or Somalian species.

00:09:11.968 --> 00:09:15.513
Adult worms are found in body cavities
and usually is asymptomatic.

00:09:18.140 --> 00:09:21.519
It is useful to compare the heads
and tails of McFlurry to help identify

00:09:21.519 --> 00:09:24.522
the species whose sugary brand cough day

00:09:24.522 --> 00:09:27.525
is 240 to 300 microns in length.

00:09:27.733 --> 00:09:30.319
The tail is pointed with no nuclei.

00:09:30.319 --> 00:09:34.282
When the sheath is present,
it usually does not stain lower.

00:09:34.282 --> 00:09:37.285
Lower is 230 to 300 microns,

00:09:37.326 --> 00:09:41.872
the toes tapered with irregularly arranged
nuclei like the tip of the tail,

00:09:42.373 --> 00:09:44.917
so I have to say
lower, lower flow of flowers.

00:09:44.917 --> 00:09:45.918
So the tip of the tail

00:09:46.961 --> 00:09:47.420
when they see

00:09:47.420 --> 00:09:50.423
this present usually does not stain.

00:09:50.506 --> 00:09:52.675
Perseus species are 125

00:09:52.675 --> 00:09:55.678
microns, so 310 microns

00:09:55.720 --> 00:09:59.181
and the tail are two nuclei,
a subchannel and terminal nuclei.

00:10:00.933 --> 00:10:02.727
The sheath, when present, may be bright

00:10:02.727 --> 00:10:05.730
pink or may not seen at all.

00:10:05.813 --> 00:10:09.525
Maserati are 160 to 240 microns.

00:10:10.860 --> 00:10:14.322
They have a long tail that are pointed
with no nuclei at the tip.

00:10:15.406 --> 00:10:18.367
The sheath is always absent.

00:10:18.367 --> 00:10:21.495
Mantle percent is 190 to 230

00:10:21.495 --> 00:10:25.708
microns, has a blunt tail with nuclei
extending all the way to the tip.

00:10:26.292 --> 00:10:29.295
The sheath is also absent.

00:10:31.213 --> 00:10:34.300
Several the images and life
cycles are courtesy of the centers

00:10:34.300 --> 00:10:37.595
for Disease Control and its laboratory
diagnostic assistance website.

00:10:37.928 --> 00:10:40.931
DPG and Travelers Health website.

00:10:41.474 --> 00:10:45.061
Other images are taken while working on
the bench at area laboratories.
