Blood Bank (Immunology)

Introduction to the ABO Blood Group

The ABO blood group system is the most important in transfusion medicine. This lecture covers basic ABO inheritance, antigen production and expression, and weak subgroups.

This video is 46m

Introduction to the Rhesus Blood Group

The Rh blood group system is one of the largest and most polymorphic. This lecture describes several Rh antigens and antibodies of clinical significance, Weak D phenotypes, and rare phenotypes that result from deletions and gene mutations. In addition, different nomenclature systems are explained.

This video is 50m

The Lewis System

The Lewis system is unique among blood group systems in that the antigens are not manufactured within the erythrocyte, nor do they form an integral part of the cytoskeletal membrane. Rather, they are synthesized by tissues, secreted into blood and body fluids, and adsorb onto the red blood cell. While antibodies against antigens in this system are fairly commonly encountered, they are generally not considered to be clinically significant in transfusion. In vitro and in vivo hemolysis are rare but have been reported. Because Lewis phenotype expression is based upon the interaction of several genes, and because the phenotype expression can be transient, the Lewis system is fascinating to learn about.

This video is 33m

Lyme Disease

This lecture focuses on how Lyme disease is transmitted, interpreting symptoms for diagnosing Lyme disease, and a discussion of associated laboratory testing.

This video is 38m

Hypersensitivities 1

In this lecture, Barbara Bonke MS, MT(ASCP)SI describes the difference between hypersensitivities and allergies, the different types of immune responses and specifics about type I hypersensitivities.

This video is 31m

Hypersensitivities 2

In this lecture, Barbara Bonke MS, MT(ASCP)SI describes type II, III and IV hypersensitivities with examples.

This video is 32m

Introduction to Antibody Identification

This lecture talks about introductory antibody identification.

This video is 17 minutes.

Transfusion Service and Blood Services Operations

This lecture talks about Transfusion Service and Blood Services Operations.

This video is 9 minutes.

SOPs for cGMPs

This lecture talks about SOPs for cGMPs.

This video is 10 minutes.

Reporting Errors

This lecture talks about Reporting Errors.

This video is 14 minutes.

Inspections of Blood Donor and Transfusion Medicine Laboratories

This lecture talks about Inspections of Blood Donor and Transfusion Medicine Laboratories.

This video is 23 minutes.

Current Good Manufacturing Practices

This lecture talks about Current Good Manufacturing Practices.

This video is 16 minutes.

Compliance and Inspections of Blood Donor and Transfusion Medicine Laboratories

This lecture talks about Compliance and Inspections of Blood Donor and Transfusion Medicine Laboratories.

This video is 9 minutes.

Change Control and Validation

This lecture talks about Change Control and Validation.

This video is 9 minutes.

How to Issue Blood Units

Blood transfusions are medical procedures used to treat cellular deficiencies and potentially life-threatening conditions such as massive blood loss, congenital disorders, malignancies, chonic anemias, and numerous other conditions. This video covers the general process for issuing blood products for infusion in patients.

This video is 5m

How to Thaw Fresh Frozen Plasma

Fresh frozen plasma, or FFP, is the liquid portion of blood that has been prepared from a whole blood donation or apheresis collection and then frozen within 8 hours of procurement. This video will show the steps for choosing and thawing plasma.

This video is 3m

How to Inspect Donor RBC and Plasma Units

This short video, created by Justin Rhees, MS, SBBCM, MLS(ASCP)^CM Assistant Professor with Weber State University, provides instructions on the proper method for inspecting donor RBC and plasma units.

This video is 2m

How to do Proper Tube Shaking Technique

This video will instruct you on the proper method to read tubes for blood banking including grading reactions.

This video is 6 minutes.

How to create reagents–Alsever's Solution

This video will instruct you on the proper method to create Alsever's Solution for use in the blood bank.

This video is 6 minutes.

Proper Use of the Sterile Tubing Welder

The collection and processing of blood products often requires manipulation and transfer of the blood products between various containers. The sterile tubing welder provides technology for connecting sterile blood containers without compromising the sterility of the fluid pathway and maintaining a functionally closed system. This tutorial will cover the sterile tubing welding process and its proper use.

Immune Thrombocytopenia

A 70-year-old male presents with on-going nosebleeds. They have a history of hypertension, diabetes mellitus type 2, gout, cronic sinusitis, aortic stenosis s/p aortic valve repair. Diagnosted with ITP ~ 5 to 10 years ago on rituximab. This presention will discuss this patient's diagnosis along with diving into immune thrombovytopenia.

This video is 16m

Autoimmune Hemolytic Anemia

An 86 year-old male, O+, with a history of anemia presented with extreme fatigue in 2020 and significant weight loss. BMBx found erythroid megloblasoid maturation and abnormal cytogenetics. Diagnosed with low-grade Myelodysplastic syndrome. Severe anemia a year later, repeated BMBx and did not met MDS criteria. This presentation will further discuss the patient's case and labs along with diving into autoimmune hemolytic anemia.

This video is 19m

Gerbich Blood Group System

A 19 year-old female who was G2P1 who was currently pregnant and was going to have another baby. She was typed B+ in 2019. In 2020, there was high incidence noted – Pan reactive with panel. In 2021 that antibody was further identified as Anti-Ge. Unknown which antigen among Ge 2/3/4 is reactive. This presentation will discuss this patient's diagnosis along with exploring Gerbich Blood Grouping.

This video is 27m

Hemolysis and Clostridium Perfringens Infection

A 65 year-old male, O+, admitted with a small bowel obstruction for 4 days. Two weeks later presented to ED with abdominal pain, chills, fatigue, etc. Lab reported hemolysis. Imaging showed hepatic abscess and cholecystitis. This presentation will further discuss the patient's labs and findings along with explore hemolysis and clostridium perfringens infection.

This video is 14m

Intrauterine RBC Transfusions

37 year-old women at 29 weeks pregnant, type A+, 4 cm placental mass, polyhydramnios, and new concern for fetal anemia based on elevated MCA dopplers was admitted to be consulted for amnioreduction and intrauterine transfusion. This presention will further discuss this case along with explore intrauterine RBC transfusions in more detail.

This video is 17m

Post-Artesunate Delayed Hemolysis

A 61 year-old male with HTN transferred from an OSH with MET, acidosis, AKI, and hypoglycemia in the setting of severe malaria and an incidental COVID infection 5 days after returning from Mazambique. No malaria prophylaxis taken. Smear showed Plasmodium falciparum, parasitemia, 24.4%, for which he was started on Artesunate q12h x3 doses, then daily until parasitemia was <1% followed by coatem BID x3 days. Post coatem, he began to feel more fatigued with intermittent mild fevers and darkening urine. He became HDS and aferbrile, but his CBC was notable for a Hgb of 5.1 with PLT of 363. This presentation will further explore this case along with discussing post-artesunate delayed hemolysis in greater detail.

This video is 21m

Case Review: HLA and Platelets

An 81 year old, B positive female with a history of hemochromatosis, multiple sclerosis, IBS and was recently diagnosed with AML presents with a WBC 2.83k/uL, Hgb 7.5 g/dL, and platelets 6,000/uL. This case review will discuss human leukocyte antigens and how HLA antibodies affect transfusions.

This video is 14m