Clinical Flow Cytometry for the Perplexed-Part 1: Introduction to Gating

 

Introduction to gating in flow cytometry with an emphasis on gating strategies in clinical flow cytometry used to identify neoplastic populations of interest. Also covers major artifacts that can lead to spurious results that should be recognized. This lecture serves as the foundation for practical immunophenotyping skills needed to succeed in your early clinical flow cytometry rotation

Originally published on February 2, 2022

Lecture Presenter

David P. Ng, MD

David P. Ng, MD

Assistant Clinical Professor
University of Utah School of Medicine
Medical Director, Hematopathology
ARUP Laboratories

Dr. David P. Ng is a medical director of hematopathology at ARUP Laboratories and an assistant clinical professor of pathology at the University of Utah. Dr. Ng received his medical degree from the University of Illinois at Chicago College of Medicine. He then completed an anatomic and clinical pathology residency at Dartmouth-Hitchcock Medical Center and a hematopathology fellowship at the University of Washington. Dr. Ng is board certified in anatomic and clinical pathology and hematology. He is the recipient of the Janis Giorgi Young Investigator award and the John H. Rippey Grant for Laboratory Quality Assurance. His research interests include minimal residual disease testing, clinical flow cytometry, and deep learning applications in flow cytometry.

Objectives

After this presentation, participants will be able to:

  • Define the parameters used in finding populations of interest
  • Understand basic gating strategies used in clinical flow cytometry
  • Recognize the difference between gating and phenotyping markers
  • Identify "junk" events and ignore them for the purposes of clinical immunophenotyping

Sponsored by:

University of Utah School of Medicine, Department of Pathology, and ARUP Laboratories