Clinical Flow Cytometry for the Perplexed-Part 2: Technical Considerations

 

This lecture covers the technology behind flow cytometry including a very high level overview of how a modern flow cytometer works, how fluorophores work along with their technical limitations such as tandem breakdown, and compensation issues. Also covered an explanation for modern displays of bivariate data, fit for purpose panel design, and more examples compensation artifacts.

Originally published on February 2, 2022

Lecture Presenter

David P. Ng, MD

David P. Ng, MD

Assistant Clinical Professor
University of Utah School of Medicine
Medical Director, Hematopathology
ARUP Laboratories

Dr. David P. Ng is a medical director of hematopathology at ARUP Laboratories and an assistant clinical professor of pathology at the University of Utah. Dr. Ng received his medical degree from the University of Illinois at Chicago College of Medicine. He then completed an anatomic and clinical pathology residency at Dartmouth-Hitchcock Medical Center and a hematopathology fellowship at the University of Washington. Dr. Ng is board certified in anatomic and clinical pathology and hematology. He is the recipient of the Janis Giorgi Young Investigator award and the John H. Rippey Grant for Laboratory Quality Assurance. His research interests include minimal residual disease testing, clinical flow cytometry, and deep learning applications in flow cytometry.

Objectives

After this presentation, participants will be able to:

  • Describe how a flow cytometer works
  • Define the pitfalls and artifacts related to Tandem Breakdowns and Spillover/Compensation

Sponsored by:

University of Utah School of Medicine, Department of Pathology, and ARUP Laboratories